OPS>SOFTWARE>DOCUMENTATION

Documentation

This page provides links to documentation for OpenPolScope software, either as downloadable PDF file or as htm link. Some of the documentation is distributed with the software and is accessible via the Help menu of the installed OpenPolScope software. In addition, this page provides Tips & Tricks of the image processing trade.

OpenPolScope Protocols

In a forthcoming article in "Current Protocols", we describe the use of the OpenPolScope software to image and measure polarized fluorescence of labeled cell structures. The following link opens the page that identifies the software versions used when the article was prepared:

Polarized Fluorescence Microscopy to Study Cytoskeleton Assembly and Organization in live cells. (Molly McQuilken et al., 2014)

OpenPolScope Instrumentation

Polarization microscopy with the LC-PolScope

A start up guide for Polarization Microscopy terms and techniques Click Here

OpenPolScope Software: Acquisition & Processing

Pol-Acquisition Manual

Pol-Acquisition Software Manual Click Here
Pol-Acquisition using Filter-Wheel instead of Liquid Crystal Click Here

Pol-Analyzer Manual

Pol-Analyzer Software Manual Click Here

OpenPolScope Software: Tools

Abrio File Opener

Abrio File Opener Manual Click Here

Orientation Lines

Orientation Lines Manual Click Here

OpenPolScope Software: Sub Plugins (Pre/Post Processing)

ColorMap

ColorMap OpenPolScope Manual Click Here

Ratioing

Ratioing OpenPolScope Manual Click Here

ImagejMacro

ImagejMacro OpenPolScope Manual Click Here

MatlabDemoPlugin

MatlabDemoPlugin OpenPolScope Manual Click Here

OpenPolScope Software: Tips & Tricks

Export Micro-Manager metadata and more

How to export Micro-Manager Metadata to an Excel spreadsheet Click Here

Export Orientation Lines

How to export Orientation Lines (Anglular information) to an Excel spreadsheet Click Here

Combining OpenPolScope Acquisition with other modalities

How to combine and Acquire other modalities (Brightfield, Darkfield, Fluorescence, etc.) along with PolScope data Click Here

Batch Processing

How to Process multiple datasets in a Session at the same time using the same parameters Click Here

Re-Ordering Processing Plugins

How to Re-Order the sequence of Pre/Post Processing Plugins Click Here

OpenPolScope Software: External Control

MatLab

Controlling LC Device via Micro-Manager from your Matlab environment Click Here

Python

Controlling LC Device via Micro-Manager from your Python environment Click Here

Other Software Environment

Controlling LC Device via Micro-Manager from Other Software environment Click Here