Since 2014, the OpenPolScope Resource at MBL provides services, hardware, and software for polarized light microscopy, complementing the software and information available on the OpenPolScope.org website.

The OPS Resource sells the OpenPolScope Hardware Kit, which consists of a liquid crystal device with digital controller and some additional optical and mechanical components (see webpage OPS Hardware). Together with the OPS Software available for free on the webpage OPS Downloads, the Hardware Kit allows the user to convert an exisiting research grade microscope into a sensitive polarized light microscope that comprehensively maps optical anisotropies (birefringence, diattenuation, and polarized fluorescence) in biological and other specimens.

The OPS Resource also provides OpenPolScope Services for installing, operating and maintaining OpenPolScope systems in laboratories around the world. In addition, we make arrangements for developing custom imaging software, in particular for the ImageJ and Micro-Manager platforms.

The following examples give an overview of microscope stands and configurations of OpenPolScope systems that are installed in MBL and collaborative laboratories and facilities.


Microscope Components Setup
Nikon Eclipse Ti
(Includes Lightfield)
  • Birefringence
  • Polarized Fluorescence
  • Diattenuation
    Related Pages:
  1. Polarized Light Field Imaging
  • Hamamatsu Flash 4 Camera
  • QImaging Retiga

    (with lenslet array)
Nikon Ti Nikon Ti Nikon Ti
Nikon Microphot
  • Birefringence
  • Polarized Fluorescence
  • Diattenuation
  • QImaging Retiga
Nikon Microphot Nikon Microphot Nikon Microphot
Zeiss Axiovert 200M
  • Birefringence
  • Polarized Fluorescence
  • Diattenuation
  • QImaging Retiga
Zeiss Axiovert 200M Zeiss Axiovert 200M Zeiss Axiovert 200M
Olympus IX-83 - MBL Summer, 2013
MultiFocus Polarization Microscope
(Sara Abrahamsson)
  • Polarized Fluorescence
    Related Pages:
  1. MultiFocus Polarization Microscope
  2. Seeing Life in 4D
  • Andor iXon-888
    (9 simultaneous focal planes)
  • Hamamatsu Orca Flash4
    (25 simultaneous focal planes)
Zeiss Axiovert - MultiFocus Polarization Microscope
Zeiss LSM 780
(Confocal LC-PolScope)
  • Polarized Fluorescence
    Related Pages:
  1. Developed by Shalin Mehta at MBL
  • laser - 458,488,516,543,633nm.
    GaAsP detector capable of
    spectral imaging
Confocal LC-PolScope (Zeiss LSM 780)
Zeiss AX10 - Gladfelter lab
(Ludl Filter Wheel based Orientation Independent PolScope)
  • Polarized Fluorescence
    Related Pages:
  1. Polarized Fluorescence Microscopy
  2. Filter-Wheel instead of Liquid Crystal
  3. Gladfelter lab (UNC, Chapel Hill)
  4. Gladfelter lab (Dartmouth College)
  • Hamamatsu
  • Ludl Filter Wheel with
    linear polarizers

Custom Olympus IX-70 Scope - Tani lab
  • Birefringence
    Related Pages:
  1. Tani Lab
  • Andor iXon Ultra
Custom Olympus Scope Custom Olympus Scope Custom Olympus Scope

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Last Page Update on August 24 2017 22:56