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OpenPolScope Multi Mode MultiD Acquisition

This section explains how to combine and Acquire other modalities (Brightfield, Darkfield, Fluorescence, etc.) along with PolScope data using the OpenPolScope software.

This guide assumes the necessary hardware has been configured using the Hardware configuration wizard.
Step 1: Start by creating a new Group using the + button and then naming eg. "Multi-D Group" and selecting all hardware that require to be changed between acquiring PolScope dataset and other type of datasets.

OpenPolScope Multi Mode MultiD Acquisition


Step 2: In this example we change the Nikon TI Filter Block. In our setup the TIFilterBlock1-Label 1 , TIFilterBlock1-State 0 houses the PolScope Analyzer.

OpenPolScope Multi Mode MultiD Acquisition


Step 3: We then proceed to define a Preset using Filter Block Label 2 or State 1 not 'Label 1' which is being used for PolScope.

OpenPolScope Multi Mode MultiD Acquisition


Step 4: The defined "Multi-D Group" will now show up.

OpenPolScope Multi Mode MultiD Acquisition


Step 5: If there are more than 1 then proceed to add a second hardware state using the + button for Preset selecting 'Label 3'

OpenPolScope Multi Mode MultiD Acquisition


Step 6: We now have defined 2 Presets for acquiring non-PolScope images.

OpenPolScope Multi Mode MultiD Acquisition


Step 7: At this point one should Save this hardware configuration using the Save button.
Step 8: Start Pol-Acquisition. Under Options in the Multi-Mode Acquisition panel enable both options.

OpenPolScope Multi Mode MultiD Acquisition


Step 9: Click on the "Series" button and the dialog below should pop-up. Select the group you want merged with PolScope acquisition if you have multiple Groups and hit OK.

OpenPolScope Multi Mode MultiD Acquisition


Step 10: You should then see the below dialog pop-up. The preset name will be populated and the only thing to be selected is the Label. Set it to 1 since 1 has the Analyzer cube. Hit OK.

OpenPolScope Multi Mode MultiD Acquisition


Step 11: If 'Series...' button was clicked previously the Multi-D dialog will show up. You can at this point change the exposure if required for the Acq-Preset 1 and 2. Setup Time and Z if required. Proceed acquisition by clicking the "Acquire!" button.
If 'SM' button is used instead of 'Series...' the following dialog does not show up and a Single time-point acquisition proceeds with the default settings.

OpenPolScope Multi Mode MultiD Acquisition


The final Acquired dataset should show a total of 9 channels (2 Acq-Presets + 7 Pol related)

OpenPolScope Multi Mode MultiD Acquisition


If you are satisfied with how you have defined your hardware for the Acquisition you may disable the option 'Always ask for Pol related Hardware Settings on Merge'
Doing so will from now on only bring up Step 9 & 11 when using the 'Series...' button and only Step 9 when using the 'SM' button.

OpenPolScope Multi Mode MultiD Acquisition


If any of the "Multi-D Group" related hardware is changed eg. Filter cube position then you will need to re-enable the above option once to redefine the position of the Analyzer.

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Last Page Update on July 16 2013 15:06